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rt recombinant dna reagent pet28a cas9  (Addgene inc)


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    Structured Review

    Addgene inc rt recombinant dna reagent pet28a cas9
    Rt Recombinant Dna Reagent Pet28a Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+recombinant+dna+reagent+pet28a+cas9/pET28a%2FCas9-Cys+(Plasmid+%2353261)/10__7554_slash_elife__35069-292-155-160
    Average 93 stars, based on 36 article reviews
    rt recombinant dna reagent pet28a cas9 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) SOX2 NA Transcript ID_ENSEMBL: ENST00000325404 Gene (Homo sapiens) SOX9 NA Transcript ID_ENSEMBL: ENST00000245479 Continued on next page Dewari et al. eLife 2018;7:e35069. .. DOI: https://doi.org/10.7554/eLife.35069 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) OLIG2 NA Transcript ID_ENSEMBL: ENST00000333337 Cell line (Mus musculus) ANS4 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) BL6 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) IENS PMID: 17936558 mouse glioma-initiating stem cells Cell line (Mus musculus) ES (Tg2a) This paper mouse embryonic stem cells Cell line (Homo sapiens) MasterShef7 (MS7) This paper human embryonic stem cells Cell line (Homo sapiens) G7 human tumour-derived This paper Cell derived from GBM tumours Antibody V5 tag eBioscience TCM5 #14-6796-82; RRID:AB_10718239 1:1000 Overnight 4 ̊C Antibody HA Tag Cell Signalling 6E2 #2367; RRID:AB_10691311 1:100 Overnight 4 ̊C Antibody FLAG tag Sigma-Aldrich #F3165; RRID:AB_259529 1:2000 Overnight 4 ̊C Antibody Myc tag Cell Signalling 9B11 #2276; RRID:AB_331783 1:4000 Overnight 4 ̊C Antibody Alexa Fluor Plus Secondary antibody Thermo Fisher Scientific Alexa Fluor Plus 647; RRID:AB_141663 1:1000 1 hr RT Recombinant DNA reagent pET28a/Cas9-Cys Addgene Addgene, #53261 Peptide, recombinant protein Cas9 protein Addgene Addgene, #53261 Chemical compound, drug crRNAs Integrated DNA Technologies, USA CRISPR-Cas9 crRNA Chemical compound, drug tracrRNAs Integrated DNA Technologies, USA CRISPR-Cas9 tracrRNA Software, algorithm Tag-IN tool This paper http://tagin.stembio.org for crRNA ssODN design Software, algorithm DESKGEN tool https://www.deskgen.com/ landing/cloud.html https://www.deskgen.com/ landing/cloud.html for crRNA ssODN design ..

    FLAG-tag:

    Article Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) SOX2 NA Transcript ID_ENSEMBL: ENST00000325404 Gene (Homo sapiens) SOX9 NA Transcript ID_ENSEMBL: ENST00000245479 Continued on next page Dewari et al. eLife 2018;7:e35069. .. DOI: https://doi.org/10.7554/eLife.35069 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) OLIG2 NA Transcript ID_ENSEMBL: ENST00000333337 Cell line (Mus musculus) ANS4 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) BL6 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) IENS PMID: 17936558 mouse glioma-initiating stem cells Cell line (Mus musculus) ES (Tg2a) This paper mouse embryonic stem cells Cell line (Homo sapiens) MasterShef7 (MS7) This paper human embryonic stem cells Cell line (Homo sapiens) G7 human tumour-derived This paper Cell derived from GBM tumours Antibody V5 tag eBioscience TCM5 #14-6796-82; RRID:AB_10718239 1:1000 Overnight 4 ̊C Antibody HA Tag Cell Signalling 6E2 #2367; RRID:AB_10691311 1:100 Overnight 4 ̊C Antibody FLAG tag Sigma-Aldrich #F3165; RRID:AB_259529 1:2000 Overnight 4 ̊C Antibody Myc tag Cell Signalling 9B11 #2276; RRID:AB_331783 1:4000 Overnight 4 ̊C Antibody Alexa Fluor Plus Secondary antibody Thermo Fisher Scientific Alexa Fluor Plus 647; RRID:AB_141663 1:1000 1 hr RT Recombinant DNA reagent pET28a/Cas9-Cys Addgene Addgene, #53261 Peptide, recombinant protein Cas9 protein Addgene Addgene, #53261 Chemical compound, drug crRNAs Integrated DNA Technologies, USA CRISPR-Cas9 crRNA Chemical compound, drug tracrRNAs Integrated DNA Technologies, USA CRISPR-Cas9 tracrRNA Software, algorithm Tag-IN tool This paper http://tagin.stembio.org for crRNA ssODN design Software, algorithm DESKGEN tool https://www.deskgen.com/ landing/cloud.html https://www.deskgen.com/ landing/cloud.html for crRNA ssODN design ..

    Recombinant:

    Article Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) SOX2 NA Transcript ID_ENSEMBL: ENST00000325404 Gene (Homo sapiens) SOX9 NA Transcript ID_ENSEMBL: ENST00000245479 Continued on next page Dewari et al. eLife 2018;7:e35069. .. DOI: https://doi.org/10.7554/eLife.35069 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) OLIG2 NA Transcript ID_ENSEMBL: ENST00000333337 Cell line (Mus musculus) ANS4 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) BL6 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) IENS PMID: 17936558 mouse glioma-initiating stem cells Cell line (Mus musculus) ES (Tg2a) This paper mouse embryonic stem cells Cell line (Homo sapiens) MasterShef7 (MS7) This paper human embryonic stem cells Cell line (Homo sapiens) G7 human tumour-derived This paper Cell derived from GBM tumours Antibody V5 tag eBioscience TCM5 #14-6796-82; RRID:AB_10718239 1:1000 Overnight 4 ̊C Antibody HA Tag Cell Signalling 6E2 #2367; RRID:AB_10691311 1:100 Overnight 4 ̊C Antibody FLAG tag Sigma-Aldrich #F3165; RRID:AB_259529 1:2000 Overnight 4 ̊C Antibody Myc tag Cell Signalling 9B11 #2276; RRID:AB_331783 1:4000 Overnight 4 ̊C Antibody Alexa Fluor Plus Secondary antibody Thermo Fisher Scientific Alexa Fluor Plus 647; RRID:AB_141663 1:1000 1 hr RT Recombinant DNA reagent pET28a/Cas9-Cys Addgene Addgene, #53261 Peptide, recombinant protein Cas9 protein Addgene Addgene, #53261 Chemical compound, drug crRNAs Integrated DNA Technologies, USA CRISPR-Cas9 crRNA Chemical compound, drug tracrRNAs Integrated DNA Technologies, USA CRISPR-Cas9 tracrRNA Software, algorithm Tag-IN tool This paper http://tagin.stembio.org for crRNA ssODN design Software, algorithm DESKGEN tool https://www.deskgen.com/ landing/cloud.html https://www.deskgen.com/ landing/cloud.html for crRNA ssODN design ..

    CRISPR:

    Article Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) SOX2 NA Transcript ID_ENSEMBL: ENST00000325404 Gene (Homo sapiens) SOX9 NA Transcript ID_ENSEMBL: ENST00000245479 Continued on next page Dewari et al. eLife 2018;7:e35069. .. DOI: https://doi.org/10.7554/eLife.35069 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) OLIG2 NA Transcript ID_ENSEMBL: ENST00000333337 Cell line (Mus musculus) ANS4 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) BL6 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) IENS PMID: 17936558 mouse glioma-initiating stem cells Cell line (Mus musculus) ES (Tg2a) This paper mouse embryonic stem cells Cell line (Homo sapiens) MasterShef7 (MS7) This paper human embryonic stem cells Cell line (Homo sapiens) G7 human tumour-derived This paper Cell derived from GBM tumours Antibody V5 tag eBioscience TCM5 #14-6796-82; RRID:AB_10718239 1:1000 Overnight 4 ̊C Antibody HA Tag Cell Signalling 6E2 #2367; RRID:AB_10691311 1:100 Overnight 4 ̊C Antibody FLAG tag Sigma-Aldrich #F3165; RRID:AB_259529 1:2000 Overnight 4 ̊C Antibody Myc tag Cell Signalling 9B11 #2276; RRID:AB_331783 1:4000 Overnight 4 ̊C Antibody Alexa Fluor Plus Secondary antibody Thermo Fisher Scientific Alexa Fluor Plus 647; RRID:AB_141663 1:1000 1 hr RT Recombinant DNA reagent pET28a/Cas9-Cys Addgene Addgene, #53261 Peptide, recombinant protein Cas9 protein Addgene Addgene, #53261 Chemical compound, drug crRNAs Integrated DNA Technologies, USA CRISPR-Cas9 crRNA Chemical compound, drug tracrRNAs Integrated DNA Technologies, USA CRISPR-Cas9 tracrRNA Software, algorithm Tag-IN tool This paper http://tagin.stembio.org for crRNA ssODN design Software, algorithm DESKGEN tool https://www.deskgen.com/ landing/cloud.html https://www.deskgen.com/ landing/cloud.html for crRNA ssODN design ..

    Software:

    Article Title: An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
    Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) SOX2 NA Transcript ID_ENSEMBL: ENST00000325404 Gene (Homo sapiens) SOX9 NA Transcript ID_ENSEMBL: ENST00000245479 Continued on next page Dewari et al. eLife 2018;7:e35069. .. DOI: https://doi.org/10.7554/eLife.35069 18 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) OLIG2 NA Transcript ID_ENSEMBL: ENST00000333337 Cell line (Mus musculus) ANS4 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) BL6 PMID: 28096221 mouse neural stem cells Cell line (Mus musculus) IENS PMID: 17936558 mouse glioma-initiating stem cells Cell line (Mus musculus) ES (Tg2a) This paper mouse embryonic stem cells Cell line (Homo sapiens) MasterShef7 (MS7) This paper human embryonic stem cells Cell line (Homo sapiens) G7 human tumour-derived This paper Cell derived from GBM tumours Antibody V5 tag eBioscience TCM5 #14-6796-82; RRID:AB_10718239 1:1000 Overnight 4 ̊C Antibody HA Tag Cell Signalling 6E2 #2367; RRID:AB_10691311 1:100 Overnight 4 ̊C Antibody FLAG tag Sigma-Aldrich #F3165; RRID:AB_259529 1:2000 Overnight 4 ̊C Antibody Myc tag Cell Signalling 9B11 #2276; RRID:AB_331783 1:4000 Overnight 4 ̊C Antibody Alexa Fluor Plus Secondary antibody Thermo Fisher Scientific Alexa Fluor Plus 647; RRID:AB_141663 1:1000 1 hr RT Recombinant DNA reagent pET28a/Cas9-Cys Addgene Addgene, #53261 Peptide, recombinant protein Cas9 protein Addgene Addgene, #53261 Chemical compound, drug crRNAs Integrated DNA Technologies, USA CRISPR-Cas9 crRNA Chemical compound, drug tracrRNAs Integrated DNA Technologies, USA CRISPR-Cas9 tracrRNA Software, algorithm Tag-IN tool This paper http://tagin.stembio.org for crRNA ssODN design Software, algorithm DESKGEN tool https://www.deskgen.com/ landing/cloud.html https://www.deskgen.com/ landing/cloud.html for crRNA ssODN design ..



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    rt recombinant dna reagent pet28a cas9  (Addgene inc)
    93
    Addgene inc rt recombinant dna reagent pet28a cas9
    Rt Recombinant Dna Reagent Pet28a Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt+recombinant+dna+reagent+pet28a+cas9/pET28a%2FCas9-Cys+(Plasmid+%2353261)/10__7554_slash_elife__35069-292-155-160
    Average 93 stars, based on 1 article reviews
    rt recombinant dna reagent pet28a cas9 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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